DNA

Part:BBa_K2927022

Designed by: KUO, PEI-CHING   Group: iGEM19_CCU_Taiwan   (2019-08-29)

Negative control sequence for crRNA 3 target

To generate negative control vector that cannot bind by crRNA-3, we mutated five nucleotides within the target region of crRNA-3 in positive control vector. Negative control vector is applied to examine the specify of Cas12a activation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None